Coding

Part:BBa_K4482015:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-10-10)


HIRMAb-FGF2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3966
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
    Illegal NotI site found at 3955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 537
    Illegal BamHI site found at 1901
    Illegal BamHI site found at 1972
    Illegal BamHI site found at 3277
    Illegal XhoI site found at 575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3966
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3966
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1636
    Illegal BsaI.rc site found at 598
    Illegal SapI.rc site found at 946


Design Notes

The DNA sequence was codon optimized for gene synthesis and for the expression in mammalian cells. The sequence for FGF2 was attached to the C-terminus of the sequence for HIRMAb.


Source

This part was engineered through PCR and Gibson clonings.


References